wpi glass pipette|WPI Glass Pipette Holder 1.0 mm, For NANOFIL Syringe : broker WPI Glass Pipette Holder. 1.0 mm, For NANOFIL Syringe. Sign In to View . Generally speaking, items should be re-sterilized after each use, but items packaged in double-wrap linen packs or an inner layer of paper and outer layer of plastic have been proven to remain sterile for up to 96 weeks.
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WPI Glass Pipette Holder. 1.0 mm, For NANOFIL Syringe. Sign In to View . A gas-tight syringe (10 μL, Hamilton) was mounted onto a microinjection pump .WPI Trough Filament Trough Filament, 2 mm wide at Sigma-Aldrich
Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto, Japan. Human Brain Research Center, Graduate School of Medicine, Kyoto University, Kyoto, Japan . with a glass pipette inclined at an angle of 25° laterally to the vertical axis. The AAV was injected at two depths (2.8 mm and 2.6 mm from the cortical dura mater, volume 100 . Briefly, borosilicate glass capillaries (catalog no. 1B100-4, outer diameter: 1.0 mm and inner diameter: 0.78 mm, WPI Europe, Berlin, Germany) were pulled (P-97 Flaming/Brown type Micropipette Puller, Sutter Instrument) to forge micropipettes whose tips were bevelled at 30° to a tip diameter of 2.5 μm.Borosilicate patch pipettes were pulled to an open-tip resistance of 2 to 4 MΩ. Signals were amplified (Axopatch 700A; Molecular Devices) and then digitized using customized routines in commercial software (IGOR Pro; WaveMetrics). Evoked synaptic responses were produced through extracellular stimulation with monopolar glass pipette electrodes .Discover a wide range of research and production products with supply security and value-added services at Fisher Scientific.
With neonatal mice, a glass pipette is inserted through the PSCC cartilage to inject. It remains . WPI) coupled to a glass pipette. Two soft microtubes were connected to each other . with a glue .
WPI Glass Pipette Holder 1.0 mm, For NANOFIL Syringe
Lower the pipette or syringe to the target using the stereotaxic arm and inject solution as for standard stereotaxic approaches. Leave in place for 1-5 min after injection, to avoid a needle track when using volumes between 3-50 nL. Then, lift the pipette or syringe using the stereotaxic arm. Repeat step 8.1. for multiple targets. 9. The virus was then injected into the brain region specifically at a speed of 30 nL min −1 using a 10 mL microsyringe with a calibrated glass microelectrode and syringe pump (1B 100–3, WPI, USA). To avoid virus leakage, the microsyringe was left at the injection site for 5–10 min after the virus injection was completed. Microinjection pipettes were made from filamented borosilicate capillaries (1B100F-4; WPI Instruments, Waltham, MA) with inner and outer diameters of 0.8 and 1.0 mm, respectively. . FRAP experiments were performed in small borosilicate glass capillaries with an inner diameter of 50 ± 2 μm (Hilgenberg, Malsfeld, Germany). Measurements and .glass microelectrodes connected to an infusion pump (Nanoliter 2010 Injector, WPI) at a rate of 50 nl/min. The pipette was left in the injection site for approximately 10 minutes following infusion to prevent virus overflow. For the MS/vDB injection, 200-300 nl AAV virus was slowly injected into the MS/vDB of ChAT-
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Patch pipettes (6–8 MΩ resistance) made from borosilicate glass (1.5 mm O.D; PG150T-15; Harvard Apparatus, UK) were filled with an internal solution containing (in mM): 127 K-gluconate, 4 NaCl . Patch pipettes (4–8 MΩ; Harvard glass) were filled with a potassium gluconate based internal solution containing (in mM): 135 C 6 H 11 KO 7, 8 NaCl, 10 HEPES, 0.1 EGTA, 2 Mg 2 ATP, 0.3 Na 3 GTP .
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Intracellular recording electrodes were formed on a Sutter Instruments P-80 micropipette puller from medium-walled glass (1BF100, WPI). Micropipettes were filled with 4 M cesium acetate (CsAc) to reduce K + conductances, 50 mM QX-314 to block Na + conductances, and 2% biocytin for intracellular labeling of recorded neurons. The electrodes were .The Axon Digidata 1440A is a high-performance data acquisition system designed for electrophysiology research. It provides precise and reliable data recording capabilities for a wide range of applications.
收集整理公开的子域名字典. Contribute to YulinSec/AwsomeSubdomainDicts development by creating an account on GitHub.Alexa Fluor 488 dye is a fluorescent label used in various biomedical applications. It has an excitation wavelength of 488 nm and an emission wavelength of 520 nm, making it compatible with common fluorescence imaging and detection instruments.Patch clamp recordings were performed with an Axopatch 200B Patch clamp (PC) amplifier (Axon Instruments) and ECS filled PC pipettes (~6 MΩ; borosilicate glass, 1.5/0.84 mm ID/OD, WPI) in a cell attached configuration (Voltage Clamp mode). Signals were digitized with a Digidata 1440A ADC (Axon Instruments) and acquired with
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Overexpression of type 1 angiotensin II receptors impairs excitation-contraction coupling in the mouse heart Katy Rivard,1,2* Scott A. Grandy,1,2* Annie Douillette,1,2 Pierre Paradis,3 Mona Nemer,4 Bruce G. Allen,1 and Céline Fiset1,2 1Research Centre, Montreal Heart Institute, Montreal, 2Faculty of Pharmacy, Université de Montréal, Montreal, 3Lady DavisPipettes were pulled from thick-walled borosilicate glass capillaries (1.5 mm outside diameter; WPI) to a tip resistance of 2–4 MΩ. The internal pipette solution contained the following (in m m): 113 K-gluconate, 4.8 MgCl 2, 4 ATP, 0.5 Tris-GTP, 14 Tris-phosphocreatine, 0.1 EGTA, and 10 HEPES, pH 7.25 with KOH, ∼290 mOsm. Reported voltages .
Epithelial cells were detached from the brush tip by gentle agitation using a sterile Pasteur pipette. Cell suspensions were . and plated on glass coverslips forming the bottom of 0.5-ml chambers that were formed using rubber seals. . O for all physiological salt solutions used. Cells were voltage clamped to 0 mV (model EVC4000, WPI), and .Axopatch 200B amplifier, Molecular Devices (5 mentions) Digidata 1550B, Molecular Devices (4 mentions) Borosilicate glass pipettes, Sutter Instruments (3 mentions) STATISTICA 64 software, StatSoft (1 mentions) SigmaPlot, Systat (1 mentions) Igor Pro 7 software, Wavemetrics (1 mentions) Igor Pro software, Wavemetrics (1 mentions) Axon 700B .Ace Glass Inc.: 1982 Ace Bulletin Vol. 22, No. 1 1996 Catalog 1600. Ace Scientific Supply Co.: 1968, Safety Issue. . 2001 Eppendorf Research =E2=84=A2 pro Electronic Pipette= s: Hands on Favorite 2002 Brinkmann BioSystems Laboratory Products and Applic= ations
The WPI recording pipettes (4–7 MΩ, USA) were filled 793 with the pipette solution containing (in mM), 100 KCl, 40 HEPES, 0.2 EGTA, 5 MgCl2, 794 2.0 Mg-ATP, 0.3 Na3-GTP, 10 phosphocreatine (pH 7.30 and 300 mOsm/kg).Discover how PubCompare.ai can help you optimize your chromic acid research. Our AI-driven protocol comparison tool allows you to easily locate and evaluate protocols from literature, pre-prints, and patents, ensuring reproducible and accruate findings. Find the best protocols and products for your chromic acid research with PubCompare.ai.The PowerLab A/D converter is a data acquisition device that converts analog signals into digital data. It can interface with a variety of sensors and transducers to capture physiological measurements. The PowerLab provides high-quality data acquisition and .
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GETINGE 733HC Steam Sterilizers are optimized for sterilization of hospital instrumentation and supplies, offering up to 20 easily accessed cycles, ideal for wrapped and unwrapped porous and non-porous hard goods, gowns or towel .
wpi glass pipette|WPI Glass Pipette Holder 1.0 mm, For NANOFIL Syringe